Thin Layer Chromatography of lipids Assignment Example | Topics and Well Written Essays - 250 words

Thin grade Chromatography of lipids - Assignment ExampleThe material pass on dissolve in the resoluteness and move up the solvent front.(a) A Rf of 1 implies that the conglomerate moved the same distance as the hexane solvent. Therefore, the analyzed compound was highly non- arctic because it travelled the same distance as hexane, which is a non-polar solvent.(b) Ethyl acetate is a polar solvent. Mixing it with hexane get out reduce the hydrophobic nature of hexane (Nollet & Toldra, 2012). Consequently, the Rf value of the same compound will reduce since it will not fully dissolve in the mixture of hexane and ethyl acetate.The TLC system for phospholipase D reaction will include the enzyme itself, phosphatidylcholine and ethyl acetate as the polar solvent. Phospholipase D will hydrolyze the phosphatidylcholine to phosphatidic acid and choline. Choline is more polar compared to phosphatidic acid hence, it will move further than the acid. Molybdenum atomizer will be used to moni tor the movement of the separated material. At 2 minute, there is itty-bitty hydrolysis, which then increases to the sixth minute. Standard phosphatidic will be used as a control in the